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What’s the difference between the pEE12.4 and pEE6.4 vectors? | |
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| The pEE12.4 vector includes the GS gene for selection of stable cell lines in glutamine-free medium. This vector is used for expression of recombinant proteins where expression of only one gene is required. In the case of antibodies, pEE12.4 is used for expression of the light chain gene. For a product that requires co-expression of a second gene, such as in the case of an antibody, the pEE6.4 accessory vector is provided. The pEE6.4 vector is identical to the pEE12.4 vector, except that it lacks the elements required for expression of the GS gene. Once the gene of interest is cloned into the pEE6.4 vector, the expression cassette can be cloned into the GS-encoding pEE12.4 vector to create a "double-gene vector" ready for transfection. |
